Improved HPLC Method for Carbohydrate-deficient Transferrin in Serum
نویسندگان
چکیده
منابع مشابه
Improved HPLC method for carbohydrate-deficient transferrin in serum.
BACKGROUND There is need for a reference method for transferrin glycoforms in serum to which routine immunologic methods for the alcohol marker carbohydrate-deficient transferrin (CDT) can be traceable. We describe an improved HPLC method for transferrin glycoforms. METHODS Transferrin was iron-saturated by mixing the serum with ferric nitrilotriacetic acid, and lipoproteins were precipitated...
متن کاملImproved capillary electrophoresis method for the determination of carbohydrate-deficient transferrin in patient sera.
Capillary zone electrophoresis (CZE) with a dynamic double coating formed by charged polymeric reagents represents an effective tool for the separation of iron-saturated transferrin (Tf) isoforms and thus the determination of carbohydrate-deficient transferrin (CDT, sum of asialo-, monosialo- and disialo-Tf in relation to total Tf) in human serum. Using the CEofix-CDT reagents, a 50 microm inne...
متن کاملPregnancy and variations of carbohydrate-deficient transferrin levels measured by the candidate reference HPLC method.
AIMS Contrasting data are available on the diagnostic accuracy of carbohydrate-deficient transferrin (CDT) during pregnancy. These differences may depend in part on how CDT was evaluated and expressed. Here, we report on variations of CDT levels in pregnant women using the high performance liquid chromatography (HPLC) candidate reference method. METHODS Alanine aminotransferase, aspartate ami...
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Interviews and history questionnaires are commonly used to aid in initial diagnosis of alcoholism and identification of individuals with suspected alcohol abuse. Two of the widespread measures used as questionnaires are by National Council on Alcoholism Committee and Michigan Alcoholism Sc~xing Test. These have their limitations e.g., only 13% of 104 admissions in general hospihal were identifi...
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We studied the possible effects on serum carbohydrate-deficient transferrin (CDT) determination by a CDTect (Pharmacia) method of serum isolation in four different types of blood-collection tubes, namely: (1) glass tubes (glass Vacutainer tubes with no additive); (2) S-Monovette Neutral tubes (plastic tubes with no additive); (3) S-Monovette Serum tubes (plastic tubes with kaolin-coated plastic...
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ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 2003
ISSN: 0009-9147,1530-8561
DOI: 10.1373/clinchem.2003.023341